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procollagen type i c peptide pip eia kit  (TaKaRa)


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    Structured Review

    TaKaRa procollagen type i c peptide pip eia kit
    Procollagen Type I C Peptide Pip Eia Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pip+eia+kit/Procollagen+Type+I+C-Peptide+(PIP)+EIA+Kit/10__3390_slash_pr14182928-120-20-27
    Average 96 stars, based on 213 article reviews
    procollagen type i c peptide pip eia kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Effective release of Eryngium maritimum L. callus extract via encapsulation in multilayered liposomes for skin delivery.
    Article Snippet: Absorbance was measured at a wavelength of 450 nm using a Thermo Scientific Multiskan GO Microplate Spectrophotometer (Fisher Scientific Ltd., Vantaa, Finland). .. The PIP EIA Kit (MK101, TAKARA, Japan) was used to perform the PIP assay, following the manufacturer’s instructions. .. The PIP EIA Kit (MK101, TAKARA, Japan) was used to perform the PIP assay, following the manufacturer’s instructions.

    Article Title: Proangiogenic and Collagen-Promoting Effects of a 70% Ethanol Extract of Grateloupia angusta in Cutaneous Wound Models.
    Article Snippet: .. HDFs were plated at a density of 5 × 104 cells per well in 96-well plates and allowed to attach, then serum-deprived for 24 h. The cells were treated with GAE (20 μg/mL and 40 μg/mL) in serum-free medium for 48 h. Supernatants were collected, clarified by centrifugation (3000 rpm, 3 min, 4 ◦C), and analyzed using a PIP EIA Kit (MK101; Takara Bio, Shiga, Japan) according to the manufacturer’s instructions. .. Absorbance was read at 450 nm using a microplate reader (Multiskan FC; Thermo Fisher Scientific). https://doi.org/10.3390/ijms27073138

    Article Title: Centella asiatica Tissue Culture-Derived Extracellular Vesicles: A Multifaceted Approach to Skincare Applications
    Article Snippet: After three washes with PBS, several different concentrations (1×10 7 , 1×10 8 , 1×10 9 ) of C. asiatica EVs and 5 ng/mL TGF-β1 (AbCam Cat. #ab50036, as positive control) were added and cultured for 24 hours. .. The level of type I procollagen in cell culture supernatants was determined using a commercial PIP EIA Kit (TAKARA Cat. # MK101) according to the manufacturer’s instruction (2 step procedure). ..

    Article Title: In Vitro Characterization of Centella asiatica Extracellular Vesicles and Their Skin Repair Effects in a UVB-Irradiated Mouse Model
    Article Snippet: .. Hs68 cells were seeded at 1 × 10 5 cells per well in 12-well plates and cultured in 10% FBS DMEM at 37 °C with 5% CO 2 for 24 h. After three washes with PBS, 1 × 10 7 , 1 × 10 8 , 1 × 10 9 particles/mL of C. asiatica EVs or 5 ng/mL TGF-β1 (positive control, AbCam Cat. #ab50036, Cambridge, UK) were added and cultured for 24 h. The level of type I procollagen in cell culture supernatants (in ng/mL) was determined using a commercial PIP EIA Kit (TAKARA Cat. # MK101, Kyoto, Japan) according to the manufacturer’s instructions (2-step procedure) using a Multiskan GO microplate reader (Thermo Fisher Scientific). .. HaCaT cells were seeded into a 96-well plate and cultured for 24 h. Cells were treated with various concentrations of C. asiatica EVs or purified water (negative control) for 24 h. After treatment, culture medium was removed and cells were washed once with PBS and lysed with SuperPrep TM cell lysis & RT Kit for qPCR (Cat. #SCQ-101, TOYOBO, Tokyo, Japan).

    Article Title: Proangiogenic and Collagen-Promoting Effects of a 70% Ethanol Extract of Grateloupia angusta in Cutaneous Wound Models
    Article Snippet: .. HDFs were plated at a density of 5 × 10 4 cells per well in 96-well plates and allowed to attach, then serum-deprived for 24 h. The cells were treated with GAE (20 μg/mL and 40 μg/mL) in serum-free medium for 48 h. Supernatants were collected, clarified by centrifugation (3000 rpm, 3 min, 4 °C), and analyzed using a PIP EIA Kit (MK101; Takara Bio, Shiga, Japan) according to the manufacturer’s instructions. .. Absorbance was read at 450 nm using a microplate reader (Multiskan FC; Thermo Fisher Scientific).

    Article Title: Effective release of Eryngium maritimum L. callus extract via encapsulation in multilayered liposomes for skin delivery
    Article Snippet: .. The PIP EIA Kit (MK101, TAKARA, Japan) was used to perform the PIP assay, following the manufacturer’s instructions. ..

    Article Title: Cosmetic composition for improving skin containing taraxacum coreanum phytoplacenta culture extract that has moisturizing and soothing effects for extremely dry skin such as atopic dermatitis, and skin barrier strengthening effect
    Article Snippet: .. Then, a portion of 20 μL of the diluted supernatant of the culture medium was taken, and it was placed in an antibody-coated microtiter plate together with 20 μL of a PIP standard in a PIP EIA Kit (Takara, Cat. MK101), and 100 μL of a peroxidase-labeled antibody solution was also added to each well and reacted at 37° C. for 3 hours. ..

    Centrifugation:

    Article Title: Proangiogenic and Collagen-Promoting Effects of a 70% Ethanol Extract of Grateloupia angusta in Cutaneous Wound Models.
    Article Snippet: .. HDFs were plated at a density of 5 × 104 cells per well in 96-well plates and allowed to attach, then serum-deprived for 24 h. The cells were treated with GAE (20 μg/mL and 40 μg/mL) in serum-free medium for 48 h. Supernatants were collected, clarified by centrifugation (3000 rpm, 3 min, 4 ◦C), and analyzed using a PIP EIA Kit (MK101; Takara Bio, Shiga, Japan) according to the manufacturer’s instructions. .. Absorbance was read at 450 nm using a microplate reader (Multiskan FC; Thermo Fisher Scientific). https://doi.org/10.3390/ijms27073138

    Article Title: Proangiogenic and Collagen-Promoting Effects of a 70% Ethanol Extract of Grateloupia angusta in Cutaneous Wound Models
    Article Snippet: .. HDFs were plated at a density of 5 × 10 4 cells per well in 96-well plates and allowed to attach, then serum-deprived for 24 h. The cells were treated with GAE (20 μg/mL and 40 μg/mL) in serum-free medium for 48 h. Supernatants were collected, clarified by centrifugation (3000 rpm, 3 min, 4 °C), and analyzed using a PIP EIA Kit (MK101; Takara Bio, Shiga, Japan) according to the manufacturer’s instructions. .. Absorbance was read at 450 nm using a microplate reader (Multiskan FC; Thermo Fisher Scientific).

    Cell Culture:

    Article Title: Centella asiatica Tissue Culture-Derived Extracellular Vesicles: A Multifaceted Approach to Skincare Applications
    Article Snippet: After three washes with PBS, several different concentrations (1×10 7 , 1×10 8 , 1×10 9 ) of C. asiatica EVs and 5 ng/mL TGF-β1 (AbCam Cat. #ab50036, as positive control) were added and cultured for 24 hours. .. The level of type I procollagen in cell culture supernatants was determined using a commercial PIP EIA Kit (TAKARA Cat. # MK101) according to the manufacturer’s instruction (2 step procedure). ..

    Article Title: In Vitro Characterization of Centella asiatica Extracellular Vesicles and Their Skin Repair Effects in a UVB-Irradiated Mouse Model
    Article Snippet: .. Hs68 cells were seeded at 1 × 10 5 cells per well in 12-well plates and cultured in 10% FBS DMEM at 37 °C with 5% CO 2 for 24 h. After three washes with PBS, 1 × 10 7 , 1 × 10 8 , 1 × 10 9 particles/mL of C. asiatica EVs or 5 ng/mL TGF-β1 (positive control, AbCam Cat. #ab50036, Cambridge, UK) were added and cultured for 24 h. The level of type I procollagen in cell culture supernatants (in ng/mL) was determined using a commercial PIP EIA Kit (TAKARA Cat. # MK101, Kyoto, Japan) according to the manufacturer’s instructions (2-step procedure) using a Multiskan GO microplate reader (Thermo Fisher Scientific). .. HaCaT cells were seeded into a 96-well plate and cultured for 24 h. Cells were treated with various concentrations of C. asiatica EVs or purified water (negative control) for 24 h. After treatment, culture medium was removed and cells were washed once with PBS and lysed with SuperPrep TM cell lysis & RT Kit for qPCR (Cat. #SCQ-101, TOYOBO, Tokyo, Japan).

    Article Title: Comparative investigation on metabolites changes in soybean leaves by ethylene and activation of collagen synthesis
    Article Snippet: Genistein and daidzein are predominant phytoestrogens for an estrogen alternative due to their higher receptor binding affinity.. The present study illustrated the effective method to produce isoflavone riched soybean leaves (IRSL).. Notably, ethylene application to soybean leaves under dark condition led a distinctive increase in genistin, daidzin, malonylgenistin and malonyldaidzin, which raised to total 25,911 μg/g of isoflavones in comparison to control leaves (> 200 μg/g).

    Positive Control:

    Article Title: In Vitro Characterization of Centella asiatica Extracellular Vesicles and Their Skin Repair Effects in a UVB-Irradiated Mouse Model
    Article Snippet: .. Hs68 cells were seeded at 1 × 10 5 cells per well in 12-well plates and cultured in 10% FBS DMEM at 37 °C with 5% CO 2 for 24 h. After three washes with PBS, 1 × 10 7 , 1 × 10 8 , 1 × 10 9 particles/mL of C. asiatica EVs or 5 ng/mL TGF-β1 (positive control, AbCam Cat. #ab50036, Cambridge, UK) were added and cultured for 24 h. The level of type I procollagen in cell culture supernatants (in ng/mL) was determined using a commercial PIP EIA Kit (TAKARA Cat. # MK101, Kyoto, Japan) according to the manufacturer’s instructions (2-step procedure) using a Multiskan GO microplate reader (Thermo Fisher Scientific). .. HaCaT cells were seeded into a 96-well plate and cultured for 24 h. Cells were treated with various concentrations of C. asiatica EVs or purified water (negative control) for 24 h. After treatment, culture medium was removed and cells were washed once with PBS and lysed with SuperPrep TM cell lysis & RT Kit for qPCR (Cat. #SCQ-101, TOYOBO, Tokyo, Japan).

    Concentration Assay:

    Article Title: Comparative investigation on metabolites changes in soybean leaves by ethylene and activation of collagen synthesis
    Article Snippet: Genistein and daidzein are predominant phytoestrogens for an estrogen alternative due to their higher receptor binding affinity.. The present study illustrated the effective method to produce isoflavone riched soybean leaves (IRSL).. Notably, ethylene application to soybean leaves under dark condition led a distinctive increase in genistin, daidzin, malonylgenistin and malonyldaidzin, which raised to total 25,911 μg/g of isoflavones in comparison to control leaves (> 200 μg/g).



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